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Differenziazione di cellule staminali pluripotenti umane in precursori di cellule beta pancreatiche in un sistema di coltura 2D
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Chapters
- 00:05Introduction
- 01:22Induction of Definitive Endoderm Differentiation in Human Pluripotent Stem Cells (hPSCs) (Stage 1)
- 02:10Immunofluorescence Analysis of hPSC-Derived Definitive Endoderm (Stage 1)
- 04:19Generation of the Primitive Gut Tube from hPSCs (Stage 2)
- 05:50Assessment of Differentiation Efficiency of Generating Pancreatic Progenitors
- 08:10Results: Comparison between Optimized and Non-Optimized Protocol for Scalable Generation of Pancreatic Progenitors from hPSCs
- 09:21Conclusion
Il presente protocollo descrive un metodo potenziato per aumentare la co-espressione dei fattori di trascrizione PDX1 e NKX6.1 nei progenitori pancreatici derivati da cellule staminali pluripotenti umane (hPSC) in monostrati planari. Ciò si ottiene reintegrando la matrice fresca, manipolando la densità cellulare e dissociando le cellule endodermiche.
