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Developmental Biology
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鸡重组肢体测定,以了解细胞分化的形态发生,图案化和早期步骤
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JoVE Journal
Developmental Biology
Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
Please note that all translations are automatically generated.
Click here for the English version.
鸡重组肢体测定,以了解细胞分化的形态发生,图案化和早期步骤
DOI:
10.3791/63183-v
•
08:08 min
•
January 12, 2022
•
Jessica Cristina Marín-Llera
,
Montse Fernández-Calderón
,
Jesús Chimal-Monroy
1
Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas
,
Universidad Nacional Autónoma de México
,
2
Departamento de Anatomía y Biología Celular and IDIVAL, Facultad de Medicina
,
Universidad de Cantabria
Chapters
00:04
Introduction
00:38
Embryo Incubation and Determination of Viability
01:06
Obtaining Limb Mesodermal Cells to Fill Ectoderms
04:11
Obtaining the Limb Ectoderms
05:21
Assembling Mesodermal Cells Inside the Ectodermal Hull
06:04
Transplantation of the Filled Ectoderm into a Host Embryo
06:51
Results: Morphology and Patterning of Recombinant Limbs
07:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
重组肢体是一种强大的实验模型,可以研究细胞分化过程和胚胎信号影响下的模式生成。该协议提出了一种详细的方法,用于生成具有鸡肢 - 中胚层细胞的重组肢体,可适应从不同生物体获得的其他细胞类型。
Tags
Chicken Recombinant Limbs
Cell Differentiation
Morphogenesis
Patterning
Embryonic Signals
In Vivo
Stem Cells
Progenitor Cells
Trypsin
Collagenase
DMEM
FBS
Cell Strainer
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