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Journal
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Immunology and Infection
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用于疫苗开发的无细胞规模生产和对来自
穆里达鲁姆衣原
体的重组主要外膜蛋白的佐剂添加
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from
Chlamydia muridarum
for Vaccine Development
Please note that all translations are automatically generated.
Click here for the English version.
用于疫苗开发的无细胞规模生产和对来自
穆里达鲁姆衣原
体的重组主要外膜蛋白的佐剂添加
DOI:
10.3791/63028-v
•
12:53 min
•
March 16, 2022
•
Sean F. Gilmore*
1
,
Wei He*
1
,
Angela C. Evans
,
Delia F. Tifrea
,
Sukumar Pal
,
Brent Segelke
,
Sandra K. G. Peters
,
B. Dillon Vannest
,
Nicholas O. Fischer
,
Amy Rasley
,
Luis M. de la Maza
,
Matthew A. Coleman
3
1
Physical and Life Sciences Directorate
,
Lawrence Livermore National Laboratory
,
2
Department of Pathology and Laboratory Medicine
,
University of California
,
3
School of Medicine, Radiation Oncology
,
University of California Davis
Chapters
00:04
Introduction
01:00
Cell‐Free Production of MOMP‐tNLPs for Subunit Vaccine Formulations
04:21
MOMP‐tNLP Purification
07:19
Western and Dot Blots and Storage
10:14
Results: SDS‐PAGE Analysis
11:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了使用商业的,无细胞的蛋白质表达试剂盒来产生纳米盘中支持的膜蛋白,该膜蛋白可用作亚单位疫苗中的抗原。
Tags
Cell-free Expression
Recombinant Protein
Membrane Protein
MOMP
Chlamydia
Vaccine Development
Adjuvant
Lipid Nanoparticle
Scalable Production
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