/
/
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
A subscription to JoVE is required to view this content. Sign in or start your free trial.
14,842 Views
•
14:45 min
•
September 16, 2012
DOI:
Summary
Automatically generated
In this report, we demonstrate the staining and analysis steps of a phenotyping assay performed on fresh whole blood to enumerate major innate and adaptive leukocyte populations. We emphasize considerations for performing these procedures in the context of a multicenter clinical trial.
Explore More Videos
Peripheral Blood Leukocyte PopulationsMulticenter Clinical TrialsWhole Blood Phenotyping AssayCryopreservationImmune Response EvaluationsGranulocytesB CellsDendritic CellsFresh Leukocytes AssayVolunteer CommitmentsTimeframesWorking HoursLaboratory PersonnelMultiple Centers11-color Antibody Staining PanelTrucount TubesPhenotype Enumeration
Read Article
Cite this Article
Hensley, T. R., Easter, A. B., Gerdts, S. E., De Rosa, S. C., Heit, A., McElrath, M. J., Andersen-Nissen, E. Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay. J. Vis. Exp. (67), e4302, doi:10.3791/4302 (2012).
Copy