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Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
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Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
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March 16, 2022
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This protocol describes using commercial, cell-free protein expression kits to produce membrane proteins supported in nanodisc that can be used as antigens in subunit vaccines.
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Gilmore, S. F., He, W., Evans, A. C., Tifrea, D. F., Pal, S., Segelke, B., Peters, S. K. G., Vannest, B. D., Fischer, N. O., Rasley, A., de la Maza, L. M., Coleman, M. A. Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development. J. Vis. Exp. (181), e63028, doi:10.3791/63028 (2022).
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