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Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

English

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11,526 Views

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09:35 min

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August 09, 2013

DOI:

10.3791/50809-v

DOI:
10.3791/50809-v

•

09:35 min
August 09, 2013

•

5 Views
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1Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases,University of Texas Medical Branch

Summary

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This method describes the use of click chemistry to measure changes in host cell transcription after infection with the Rift Valley fever virus (RVFV) strain MP-12. Results can be visualized qualitatively via fluorescence microscopy or obtained quantitatively through flow cytometry. This method is adaptable for use with other viruses.

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Cite this Article

Kalveram, B., Lihoradova, O., Indran, S. V., Head, J. A., Ikegami, T. Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis. J. Vis. Exp. (78), e50809, doi:10.3791/50809 (2013).

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