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Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

DOI:
10.3791/50809-v

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09:35 min

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August 09, 2013

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, , , ,
1Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases,University of Texas Medical Branch

Chapters

  • 00:05Title
  • 01:16Analysis of Transcription by Fluorescence Microscopy
  • 04:16Analysis of Transcription by Flow Cytometry
  • 06:08Infecting 293 Cells with MP-12
  • 08:52Conclusion

Summary

Automatic Translation

Automatic Translation

This method describes the use of click chemistry to measure changes in host cell transcription after infection with the Rift Valley fever virus (RVFV) strain MP-12. Results can be visualized qualitatively via fluorescence microscopy or obtained quantitatively through flow cytometry. This method is adaptable for use with other viruses.

Tags

Click ChemistryViral InfectionHost-cell RNA SynthesisTranscriptional SuppressionRift Valley Fever Virus5-ethynyluridineFluorescent AzideFlow CytometryFluorescence Microscopy

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