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Sitokin Diferansiye İnsan Primer T Hücrelerinde Mitokondriyal Solunumun Gerçek Zamanlı İzlenmesi
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
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Sitokin Diferansiye İnsan Primer T Hücrelerinde Mitokondriyal Solunumun Gerçek Zamanlı İzlenmesi

Türkçe

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3,554 Views

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06:55 min

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October 19, 2021

DOI:

10.3791/62984-v

DOI:
10.3791/62984-v

•

06:55 min
October 19, 2021

•

3 Views
, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Summary

Automatically generated

Metabolik adaptasyon, farklılaşmayı, kalıcılığı ve sitotoksisiteyi belirlediği için T hücreleri için temeldir. Burada, ex vivo sitokin farklılaşmış insan primer T hücrelerinde mitokondriyal solunumu izlemek için optimize edilmiş bir protokol sunulmaktadır.

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Cite this Article

Mølgaard, K., Rahbech, A., Met, Ö., Svane, I. M., thor Straten, P., Desler, C., Peeters, M. J. W. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. (176), e62984, doi:10.3791/62984 (2021).

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