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Monitoreo en tiempo real de la respiración mitocondrial en células T primarias humanas diferenciadas por citoquinas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
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Monitoreo en tiempo real de la respiración mitocondrial en células T primarias humanas diferenciadas por citoquinas

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3,554 Views

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06:55 min

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October 19, 2021

DOI:

10.3791/62984-v

DOI:
10.3791/62984-v

•

06:55 min
October 19, 2021

•

3 Views
, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Summary

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La adaptación metabólica es fundamental para las células T, ya que dicta la diferenciación, la persistencia y la citotoxicidad. Aquí, se presenta un protocolo optimizado para monitorear la respiración mitocondrial en células T primarias humanas diferenciadas por citoquinas ex vivo .

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Cite this Article

Mølgaard, K., Rahbech, A., Met, Ö., Svane, I. M., thor Straten, P., Desler, C., Peeters, M. J. W. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. (176), e62984, doi:10.3791/62984 (2021).

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