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Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
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Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
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October 19, 2021
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Metabolic adaptation is fundamental for T cells as it dictates differentiation, persistence, and cytotoxicity. Here, an optimized protocol for monitoring mitochondrial respiration in ex vivo cytokine-differentiated human primary T cells is presented.
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Mølgaard, K., Rahbech, A., Met, Ö., Svane, I. M., thor Straten, P., Desler, C., Peeters, M. J. W. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. (176), e62984, doi:10.3791/62984 (2021).
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