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Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

3,554 Views

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06:55 min

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October 19, 2021

DOI:

10.3791/62984-v

DOI:
10.3791/62984-v

•

06:55 min
October 19, 2021

•

3 Views
, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

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Metabolic adaptation is fundamental for T cells as it dictates differentiation, persistence, and cytotoxicity. Here, an optimized protocol for monitoring mitochondrial respiration in ex vivo cytokine-differentiated human primary T cells is presented.

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Mølgaard, K., Rahbech, A., Met, Ö., Svane, I. M., thor Straten, P., Desler, C., Peeters, M. J. W. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. (176), e62984, doi:10.3791/62984 (2021).

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