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Real-time monitoring van mitochondriale ademhaling in cytokine-gedifferentieerde menselijke primaire T-cellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
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Real-time monitoring van mitochondriale ademhaling in cytokine-gedifferentieerde menselijke primaire T-cellen

Nederlands

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3,554 Views

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06:55 min

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October 19, 2021

DOI:

10.3791/62984-v

DOI:
10.3791/62984-v

•

06:55 min
October 19, 2021

•

3 Views
, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Summary

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Metabole aanpassing is fundamenteel voor T-cellen omdat het differentiatie, persistentie en cytotoxiciteit dicteert. Hier wordt een geoptimaliseerd protocol gepresenteerd voor het monitoren van mitochondriale ademhaling in ex vivo cytokine-gedifferentieerde menselijke primaire T-cellen.

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Cite this Article

Mølgaard, K., Rahbech, A., Met, Ö., Svane, I. M., thor Straten, P., Desler, C., Peeters, M. J. W. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. (176), e62984, doi:10.3791/62984 (2021).

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