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Journal
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Genetics
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使用短读和长读测序技术生成尿细菌完整基因组的杂交从
头
基因组组装
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Hybrid
De Novo
Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Please note that all translations are automatically generated.
Click here for the English version.
使用短读和长读测序技术生成尿细菌完整基因组的杂交从
头
基因组组装
DOI:
10.3791/62872-v
•
12:08 min
•
August 20, 2021
•
Belle M. Sharon
,
Neha V. Hulyalkar
,
Vivian H. Nguyen
,
Philippe E. Zimmern
,
Kelli L. Palmer
,
Nicole J. De Nisco
1
Department of Biological Sciences
,
University of Texas at Dallas
,
2
Department of Urology
,
University of Texas Southwestern Medical Center
Chapters
00:04
Introduction
01:00
dsDNA Pool Cleanup and Concentration
03:25
Sequence the Genome Library
05:14
Long (MinION) Reads
06:55
Generating Hybrid Genome Assembly
08:34
Assessing Assembly Quality
09:34
Genome Annotation
10:07
Results: Analysis of Extracted DNA and Complete Genome Sequencing of Urinary Bacteria
11:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议详细介绍了尿细菌培养,测序和
从头
杂交基因组组装的综合方法。它为生成完整的圆形基因组序列提供了一种可重复的程序,可用于研究有助于尿定植、发病机制和抗菌素耐药性传播的染色体和染色体外遗传元件。
Tags
Hybrid Genome Assembly
Urinary Bacteria
Short-read Sequencing
Long-read Sequencing
Complete Genomes
Genome Architecture
Mobile Genetic Elements
Culturable Bacteria
Genome Content
Adapter Ligation
Flow Cell Priming
DNA Library Preparation
Sequencing Run
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