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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

4,297 Views

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12:08 min

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August 20, 2021

DOI:

10.3791/62872-v

DOI:
10.3791/62872-v

•

12:08 min
August 20, 2021

•

2 Views
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1Department of Biological Sciences,University of Texas at Dallas, 2Department of Urology,University of Texas Southwestern Medical Center

Tags

Hybrid Genome AssemblyUrinary BacteriaShort-read SequencingLong-read SequencingComplete GenomesGenome ArchitectureMobile Genetic ElementsCulturable BacteriaGenome ContentAdapter LigationFlow Cell PrimingDNA Library PreparationSequencing Run

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Sharon, B. M., Hulyalkar, N. V., Nguyen, V. H., Zimmern, P. E., Palmer, K. L., De Nisco, N. J. Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies. J. Vis. Exp. (174), e62872, doi:10.3791/62872 (2021).

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