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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
JoVE Journal
Genetics
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JoVE Journal Genetics
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

English

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4,469 Views

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12:08 min

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August 20, 2021

DOI:

10.3791/62872-v

DOI:
10.3791/62872-v

•

12:08 min
August 20, 2021

•

39 Views
, , , , ,
1Department of Biological Sciences,University of Texas at Dallas, 2Department of Urology,University of Texas Southwestern Medical Center

Summary

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This protocol details a comprehensive approach for the culturing, sequencing, and de novo hybrid genome assembly of urinary bacteria. It provides a reproducible procedure for the generation of complete, circular genome sequences useful in studying both chromosomal and extrachromosomal genetic elements contributing to urinary colonization, pathogenesis, and antimicrobial resistance dissemination.

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Cite this Article

Sharon, B. M., Hulyalkar, N. V., Nguyen, V. H., Zimmern, P. E., Palmer, K. L., De Nisco, N. J. Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies. J. Vis. Exp. (174), e62872, doi:10.3791/62872 (2021).

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