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Immunology and Infection

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用户友好型、高通量和全自动数据采集软件，用于单颗粒冷冻电子显微镜

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

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用户友好型、高通量和全自动数据采集软件，用于单颗粒冷冻电子显微镜

DOI:

10.3791/62832-v

•

07:56 min

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July 29, 2021

•

Anil Kumar, Surekha P., Sahil Gulati, Somnath Dutta

¹Molecular Biophysics Unit,Indian Institute of Science, ²Gatan Inc.

Chapters

00:04Introduction
00:40Data Acquisition with Latitude-S
03:34Fine Alignment
04:20Data Acquisition Procedure
05:36Results: Automated Data Acquisition Using Latitude-S for Single-Particle Cryo-Electron Microscopy
07:31Conclusion

Summary

Automatic Translation

单颗粒冷冻电子显微镜需要合适的软件包和用户友好的管道来实现高通量自动数据采集。在这里，我们介绍了全自动图像采集软件包Latitude-S的应用，以及用于在低剂量条件下收集玻化生物分子数据的实用管道。

Tags

1. Automated Data Acquisition Software2. Single-particle Cryo-electron Microscopy3. Structural Characterization4. Biological Macromolecules5. Pathogen Biology6. Latitude-S7. Technique Manager8. Single-Particle Automated Data Collection9. TEM Condition10. Camera Condition11. Atlas State12. Grid State13. Hole State14. Focus State15. Data State16. Defocus Values17. Microscope Settings18. Imaging Optics19. Illumination Conditions20. Camera Exposure Time

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