Journal
/
Immunology and Infection
/
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

English

Automatically Generated

3,162 Views

•

07:56 min

•

July 29, 2021

DOI:

10.3791/62832-v

DOI:
10.3791/62832-v

•

07:56 min
July 29, 2021

•

16 Views
, , ,
1Molecular Biophysics Unit,Indian Institute of Science, 2Gatan Inc.

Summary

Automatically generated

Single-particle cryo-electron microscopy demands a suitable software package and user-friendly pipeline for high-throughput automatic data acquisition. Here, we present the application of a fully automated image acquisition software package, Latitude-S, and a practical pipeline for data collection of vitrified biomolecules under low-dose conditions.

Explore More Videos

1. Automated Data Acquisition Software2. Single-particle Cryo-electron Microscopy3. Structural Characterization4. Biological Macromolecules5. Pathogen Biology6. Latitude-S7. Technique Manager8. Single-Particle Automated Data Collection9. TEM Condition10. Camera Condition11. Atlas State12. Grid State13. Hole State14. Focus State15. Data State16. Defocus Values17. Microscope Settings18. Imaging Optics19. Illumination Conditions20. Camera Exposure Time

Read Article

Cite this Article

Kumar, A., P., S., Gulati, S., Dutta, S. User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy. J. Vis. Exp. (173), e62832, doi:10.3791/62832 (2021).

Download .ris file

Copy

Share Video

.

Copy