Journal
/
Immunology and Infection
/
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

English

Automatically Generated

8,664 Views

•

06:55 min

•

November 07, 2018

DOI:

10.3791/58200-v

DOI:
10.3791/58200-v

•

06:55 min
November 07, 2018

•

2 Views
, , , ,
1State Key Laboratory of Diarrhea Disease Detection,Zhuhai International Travel Healthcare Center

Summary

Automatically generated

Salmonella spp./Shigella spp. are common pathogens attributed to diarrhea. Here, we describe a high-throughput platform for the screening of Salmonella spp./Shigella spp. using real-time PCR combined with guided culture.

Explore More Videos

SalmonellaShigellaHigh-throughput ScreeningNAATReal-time PCRCulture IdentificationPathogensVibrioStaphylococcusE. ColiPre-enrichmentCentrifugationDNA ExtractionReal-time PCRSelenite Crystal MediumColony SelectionAutomated Microbial IdentificationO-antigen CharacterizationH-antigen Characterization

Read Article

Cite this Article

Yang, Z., Chen, X., Tu, C., Su, Y., Wang, H. A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.. J. Vis. Exp. (141), e58200, doi:10.3791/58200 (2018).

Download .ris file

Copy

Share Video

.

Copy