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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

3,162 Views

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07:56 min

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July 29, 2021

DOI:

10.3791/62832-v

DOI:
10.3791/62832-v

•

07:56 min
July 29, 2021

•

16 Views
, , ,
1Molecular Biophysics Unit,Indian Institute of Science, 2Gatan Inc.

Summary

Automatically generated

Single-particle cryo-electron microscopy demands a suitable software package and user-friendly pipeline for high-throughput automatic data acquisition. Here, we present the application of a fully automated image acquisition software package, Latitude-S, and a practical pipeline for data collection of vitrified biomolecules under low-dose conditions.

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1. Automated Data Acquisition Software2. Single-particle Cryo-electron Microscopy3. Structural Characterization4. Biological Macromolecules5. Pathogen Biology6. Latitude-S7. Technique Manager8. Single-Particle Automated Data Collection9. TEM Condition10. Camera Condition11. Atlas State12. Grid State13. Hole State14. Focus State15. Data State16. Defocus Values17. Microscope Settings18. Imaging Optics19. Illumination Conditions20. Camera Exposure Time

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Cite this Article

Kumar, A., P., S., Gulati, S., Dutta, S. User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy. J. Vis. Exp. (173), e62832, doi:10.3791/62832 (2021).

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