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High-throughput Gene Tagging in Trypanosoma brucei
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Gene Tagging in Trypanosoma brucei

High-throughput Gene Tagging in Trypanosoma brucei

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11:26 min

August 12, 2016

DOI:

11:26 min
August 12, 2016

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Summary

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Addition of a tag to a protein is a powerful way of gaining insight into its function. Here, we describe a protocol to endogenously tag hundreds of Trypanosoma brucei proteins in parallel such that genome scale tagging is achievable.

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