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Medindo o Turnover Lipídico da Membrana com o Análogo Lipídico Fluorescente Sensível ao pH ND6
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Neuroscience
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JoVE Journal Neuroscience
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Medindo o Turnover Lipídico da Membrana com o Análogo Lipídico Fluorescente Sensível ao pH ND6

português

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1,442 Views

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08:31 min

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July 29, 2021

DOI:

10.3791/62717-v

DOI:
10.3791/62717-v

•

08:31 min
July 29, 2021

•

1 Views
, , , , ,
1The Brain Institute at Florida Atlantic University, 2Department of Biomedical Sciences,Florida Atlantic University, 3Department of Chemistry and Biochemistry,Florida Atlantic University

Summary

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Este protocolo apresenta um método de imagem por fluorescência que utiliza uma classe de fluoróforos lipídicos sensíveis ao pH para monitorar o tráfego de membrana lipídica durante a exocitose celular e o ciclo de endocitose.

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Cite this Article

Alamgir, S., Pelletier, O. B., Thomas, D., Rubio, V., Stawikowski, M. J., Zhang, Q. Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6. J. Vis. Exp. (173), e62717, doi:10.3791/62717 (2021).

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