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Mesure du renouvellement lipidique membranaire avec l’analogue lipidique fluorescent ND6 sensible au pH
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Neuroscience
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JoVE Journal Neuroscience
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
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Mesure du renouvellement lipidique membranaire avec l’analogue lipidique fluorescent ND6 sensible au pH

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1,442 Views

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08:31 min

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July 29, 2021

DOI:

10.3791/62717-v

DOI:
10.3791/62717-v

•

08:31 min
July 29, 2021

•

1 Views
, , , , ,
1The Brain Institute at Florida Atlantic University, 2Department of Biomedical Sciences,Florida Atlantic University, 3Department of Chemistry and Biochemistry,Florida Atlantic University

Summary

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Ce protocole présente une méthode d’imagerie par fluorescence qui utilise une classe de fluorophores lipidiques sensibles au pH pour surveiller le trafic des membranes lipidiques pendant l’exocytose cellulaire et le cycle d’endocytose.

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Cite this Article

Alamgir, S., Pelletier, O. B., Thomas, D., Rubio, V., Stawikowski, M. J., Zhang, Q. Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6. J. Vis. Exp. (173), e62717, doi:10.3791/62717 (2021).

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