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JoVE Journal Neuroscience
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
 

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Article DOI: 10.3791/62717-v • 08:31 min • July 29th, 2021
July 29th, 2021 •
1,2, 1,2, 3, 3, 3, 1,2
1The Brain Institute at Florida Atlantic University, 2Department of Biomedical Sciences, Florida Atlantic University, 3Department of Chemistry and Biochemistry, Florida Atlantic University

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résumé
Automatic Translation

This protocol presents a fluorescence imaging method that uses a class of pH-sensitive lipid fluorophores to monitor lipid membrane trafficking during cell exocytosis and the endocytosis cycle.

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Membrane Lipid Turnover PH-sensitive Fluorescent Lipid Analog ND6Exo-/endocytosis Biomolecule Exchange Specialized Cells Insulin Secretion Neurotransmitter Release Exo-/endocytosis Research Gene And Protein Level Membrane Lipid Recycling Plasma Membrane Secretory Vesicles Lipid Distribution Intracellular Membrane Compartments Lipid Turnover Rate Biological Research Fields
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