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Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
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Neurociência
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JoVE Journal Neurociência
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

1,442 Views

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08:31 min

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July 29, 2021

DOI:

10.3791/62717-v

DOI:
10.3791/62717-v

•

08:31 min
July 29, 2021

•

1 Views
, , , , ,
1The Brain Institute at Florida Atlantic University, 2Department of Biomedical Sciences,Florida Atlantic University, 3Department of Chemistry and Biochemistry,Florida Atlantic University

Summary

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This protocol presents a fluorescence imaging method that uses a class of pH-sensitive lipid fluorophores to monitor lipid membrane trafficking during cell exocytosis and the endocytosis cycle.

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Cite this Article

Alamgir, S., Pelletier, O. B., Thomas, D., Rubio, V., Stawikowski, M. J., Zhang, Q. Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6. J. Vis. Exp. (173), e62717, doi:10.3791/62717 (2021).

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