Dergi
/
Nörobilim
/
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

1,442 Views

•

08:31 min

•

July 29, 2021

DOI:

10.3791/62717-v

DOI:
10.3791/62717-v

•

08:31 min
July 29, 2021

•

1 Views
, , , , ,
1The Brain Institute at Florida Atlantic University, 2Biyomedikal Bilimler bölümü,Florida Atlantic University, 3Department of Chemistry and Biochemistry,Florida Atlantic University

Özet

Automatically generated

This protocol presents a fluorescence imaging method that uses a class of pH-sensitive lipid fluorophores to monitor lipid membrane trafficking during cell exocytosis and the endocytosis cycle.

Explore More Videos

Membrane Lipid TurnoverPH-sensitive Fluorescent Lipid AnalogND6ExocytosisEndocytosisLive Cell Fluorescence ImagingCell MembraneMembrane TraffickingLipid MimeticPiperazine Head GroupFluorescence IntensityProtonationDeprotonationCell BiologyBiomedical Sciences

Read Article

Cite this Article

Alamgir, S., Pelletier, O. B., Thomas, D., Rubio, V., Stawikowski, M. J., Zhang, Q. Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6. J. Vis. Exp. (173), e62717, doi:10.3791/62717 (2021).

Download .ris file

Copy

Share Video

.

Copy