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Un método automatizado de puntuación microscópica para el ensayo de focos γ-H2AX en linfocitos de sangre periférica humana
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
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Un método automatizado de puntuación microscópica para el ensayo de focos γ-H2AX en linfocitos de sangre periférica humana

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4,305 Views

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08:23 min

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December 25, 2021

DOI:

10.3791/62623-v

DOI:
10.3791/62623-v

•

08:23 min
December 25, 2021

•

5 Views
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1Radiation Biophysics Division, Nuclear Medicine Department,iThemba LABS, 2Department of Biotechnology,University of the Western Cape, 3Department of Medical Biosciences,University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences,Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine,University of the Republic, 6MetaSystems Hard & Software GmbH

Summary

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Este protocolo presenta la preparación de diapositivas y la puntuación automatizada del ensayo de focos γ-H2AX en linfocitos de sangre periférica. Para ilustrar el método y la sensibilidad del ensayo, se irradiaron linfocitos aislados in vitro. Este método automatizado de detección de ADN DSB es útil para aplicaciones de dosimetría biológica rápidas y de alto rendimiento.

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Nair, S., Cairncross, S., Miles, X., Engelbrecht, M., du Plessis, P., Bolcaen, J., Fisher, R., Ndimba, R., Cunningham, C., Martínez-López, W., Schunck, C., Vandevoorde, C. An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes. J. Vis. Exp. (178), e62623, doi:10.3791/62623 (2021).

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