Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / An Automated Microscopic Scoring Method for the…
JoVE Journal
Cancer Research
Author Produced

Esto contenido es Free Access.

JoVE Journal Cancer Research
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
 

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

Article DOI: 10.3791/62623-v • 08:23 min • December 25th, 2021
December 25th, 2021 •
1, 2, 1, 3, 1, 1, 1, 1, 4, 5, 6, 1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH

Capítulos

Resumen
Automatic Translation

This protocol presents the slide preparation and automated scoring of the γ-H2AX foci assay on peripheral blood lymphocytes. To illustrate the method and sensitivity of the assay, isolated lymphocytes were irradiated in vitro. This automated method of DNA DSB detection is useful for fast and high-throughput biological dosimetry applications.

Tags

Keywords: - Automated Microscopic Scoring - γ-H2AX Foci Assay - Human Peripheral Blood Lymphocytes - Ionizing Radiation - DNA Double-strand Breaks - H2AX Phosphorylation - Automated Slide Scanning - Fluorescent Microscopy - Lymphocyte Isolation - Density Gradient Centrifugation - Gamma Irradiation - Cytocentrifugation - Standardized Slide Preparation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter