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Een geautomatiseerde microscopische scoringsmethode voor de γ-H2AX foci assay in menselijke perifere bloedlymfocyten
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
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Een geautomatiseerde microscopische scoringsmethode voor de γ-H2AX foci assay in menselijke perifere bloedlymfocyten

Nederlands

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4,365 Views

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08:23 min

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December 25, 2021

DOI:

10.3791/62623-v

DOI:
10.3791/62623-v

•

08:23 min
December 25, 2021

•

5 Views
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1Radiation Biophysics Division, Nuclear Medicine Department,iThemba LABS, 2Department of Biotechnology,University of the Western Cape, 3Department of Medical Biosciences,University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences,Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine,University of the Republic, 6MetaSystems Hard & Software GmbH

Summary

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Dit protocol presenteert de diavoorbereiding en geautomatiseerde scoring van de γ-H2AX foci assay op perifere bloedlymfocyten. Om de methode en gevoeligheid van de test te illustreren, werden geïsoleerde lymfocyten in vitrobestraald . Deze geautomatiseerde methode van DNA DSB-detectie is nuttig voor snelle en high-throughput biologische dosimetrietoepassingen.

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Cite this Article

Nair, S., Cairncross, S., Miles, X., Engelbrecht, M., du Plessis, P., Bolcaen, J., Fisher, R., Ndimba, R., Cunningham, C., Martínez-López, W., Schunck, C., Vandevoorde, C. An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes. J. Vis. Exp. (178), e62623, doi:10.3791/62623 (2021).

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