Journal
/
Cancer Research
/
Eine automatisierte mikroskopische Scoring-Methode für den γ-H2AX-Foci-Assay in humanen peripheren Blutlymphozyten
JoVE Journal
Cancer Research
Author Produced
This content is Free Access.
JoVE Journal Cancer Research
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
Please note that all translations are automatically generated. Click here for the English version.

Eine automatisierte mikroskopische Scoring-Methode für den γ-H2AX-Foci-Assay in humanen peripheren Blutlymphozyten

Deutsch

Automatically Generated

4,371 Views

•

08:23 min

•

December 25, 2021

DOI:

10.3791/62623-v

DOI:
10.3791/62623-v

•

08:23 min
December 25, 2021

•

5 Views
, , , , , , , , , , ,
1Radiation Biophysics Division, Nuclear Medicine Department,iThemba LABS, 2Department of Biotechnology,University of the Western Cape, 3Department of Medical Biosciences,University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences,Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine,University of the Republic, 6MetaSystems Hard & Software GmbH

Summary

Automatically generated

Dieses Protokoll präsentiert die Objektträgervorbereitung und das automatisierte Scoring des γ-H2AX-Foci-Assays an peripheren Blutlymphozyten. Um die Methode und Sensitivität des Assays zu veranschaulichen, wurden isolierte Lymphozyten in vitrobestrahlt. Diese automatisierte Methode des DNA-DSB-Nachweises ist nützlich für schnelle und biologische Dosimetrieanwendungen mit hohem Durchsatz.

Explore More Videos

– Automated Microscopic Scoring- γ-H2AX Foci Assay- Human Peripheral Blood Lymphocytes- Ionizing Radiation- DNA Double-strand Breaks- H2AX Phosphorylation- Automated Slide Scanning- Fluorescent Microscopy- Lymphocyte Isolation- Density Gradient Centrifugation- Gamma Irradiation- Cytocentrifugation- Standardized Slide Preparation

Read Article

Cite this Article

Nair, S., Cairncross, S., Miles, X., Engelbrecht, M., du Plessis, P., Bolcaen, J., Fisher, R., Ndimba, R., Cunningham, C., Martínez-López, W., Schunck, C., Vandevoorde, C. An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes. J. Vis. Exp. (178), e62623, doi:10.3791/62623 (2021).

Download .ris file

Copy

Share Video

.

Copy