Journal
/
/
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
JoVE Journal
Krebsforschung
Author Produced
This content is Free Access.
JoVE Journal Krebsforschung
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

4,312 Views

08:23 min

December 25, 2021

DOI:

08:23 min
December 25, 2021

5 Views
, , , , , , , , , , ,

Summary

Automatically generated

This protocol presents the slide preparation and automated scoring of the γ-H2AX foci assay on peripheral blood lymphocytes. To illustrate the method and sensitivity of the assay, isolated lymphocytes were irradiated in vitro. This automated method of DNA DSB detection is useful for fast and high-throughput biological dosimetry applications.

Read Article