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Generación de organoides pulmonares enteros en 3D a partir de células madre pluripotentes inducidas para modelar la biología y la enfermedad del desarrollo pulmonar
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
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Generación de organoides pulmonares enteros en 3D a partir de células madre pluripotentes inducidas para modelar la biología y la enfermedad del desarrollo pulmonar

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7,172 Views

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09:45 min

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April 12, 2021

DOI:

10.3791/62456-v

DOI:
10.3791/62456-v

•

09:45 min
April 12, 2021

•

18 Views
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1Department of Pediatrics,University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute

Summary

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El artículo describe la diferenciación dirigida paso a paso de células madre pluripotentes inducidas a organoides pulmonares enteros tridimensionales que contienen células pulmonares epiteliales proximales y distales junto con mesénquima.

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Cite this Article

Leibel, S. L., McVicar, R. N., Winquist, A. M., Snyder, E. Y. Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease. J. Vis. Exp. (170), e62456, doi:10.3791/62456 (2021).

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