Rivista
/
Biologia dello sviluppo
/
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

7,213 Views

•

09:45 min

•

April 12, 2021

DOI:

10.3791/62456-v

DOI:
10.3791/62456-v

•

09:45 min
April 12, 2021

•

18 Views
, , ,
1Department of Pediatrics,University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute

Summary

Automatically generated

The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.

Explore More Videos

3D Whole Lung OrganoidsInduced Pluripotent Stem CellsLung Developmental BiologyLung Disease ModelingCryopreservationGenetic ManipulationDefinitive Endoderm InductionROCK InhibitorGFR Basement Membrane Matrix

Read Article

Cite this Article

Leibel, S. L., McVicar, R. N., Winquist, A. M., Snyder, E. Y. Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease. J. Vis. Exp. (170), e62456, doi:10.3791/62456 (2021).

Download .ris file

Copy

Share Video

.

Copy