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Geração de organoides pulmonares 3D inteiros de células-tronco pluripotentes induzidas para modelagem da biologia e doença do desenvolvimento pulmonar
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Geração de organoides pulmonares 3D inteiros de células-tronco pluripotentes induzidas para modelagem da biologia e doença do desenvolvimento pulmonar

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7,213 Views

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09:45 min

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April 12, 2021

DOI:

10.3791/62456-v

DOI:
10.3791/62456-v

•

09:45 min
April 12, 2021

•

18 Views
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1Department of Pediatrics,University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute

Summary

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O artigo descreve a diferenciação direcionada de células-tronco pluripotentes induzidas a organoides pulmonares inteiros tridimensionais contendo células pulmonares epiteliais proximais e disais, juntamente com mesenchyme.

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Cite this Article

Leibel, S. L., McVicar, R. N., Winquist, A. M., Snyder, E. Y. Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease. J. Vis. Exp. (170), e62456, doi:10.3791/62456 (2021).

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