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Produzione di anticorpi monoclonali contro l'aminopeptidasi N nell'epitelio della mucosa intestinale suina
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
DOI:

09:45 min

May 18, 2021

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Chapters

  • 00:04Introduction
  • 01:04Hybridoma Technology to Produce Monoclonal Antibodies (mAbs) Against Aminopeptidase N (APN)
  • 04:20Expression of Recombinant Antibodies (rAbs) Against APN
  • 06:25Results: Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
  • 09:13Conclusion

Summary

Automatic Translation

La proteina anticorpale ricombinante espressa nelle cellule pIRES2-ZSGreen1-rAbs-APN-CHO e negli anticorpi monoclonali prodotti utilizzando la tecnologia tradizionale dell'ibridoma possono riconoscere e legarsi alla proteina N dell'aminopeptidasi suina (APN).

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