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Immunology and Infection
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Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

DOI:
10.3791/54486-v

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12:03 min

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September 05, 2016

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, , , ,
1Virus-Cell Interactions Laboratory,Lady Davis Institute for Medical Research, 2Department of Microbiology & Immunology,McGill University, 3Department of Medicine,McGill University

Chapters

  • 00:05Title
  • 00:56Cells and Transfections
  • 03:46Viral Production Assay
  • 05:38Cell Viability Assay
  • 06:28Immune Activation Assay
  • 08:59Results: Evaluation of siRNA Targeting HIV-1 RNA
  • 10:34Conclusion

Summary

Automatic Translation

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Methods to evaluate the efficacy and toxicity of RNA molecules targeting post-integration steps of the HIV-1 replication cycle are described. These methods are useful for screening new molecules and optimizing the format of existing ones.

Tags

RNAHIV-1Gene TherapyDrug TherapyEfficacyToxicityShort Hairpin RNASiRNARibozymeRNA Decoy293T CellsViral ProductionCell ViabilityImmune Activation

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