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In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
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Immunology and Infection
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JoVE Journal Immunology and Infection
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

English

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17,240 Views

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14:14 min

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January 06, 2014

DOI:

10.3791/50823-v

DOI:
10.3791/50823-v

•

14:14 min
January 06, 2014

•

4 Views
, , ,
1Department of Pediatrics,Harvard Medical School, 2Department of Pediatric Gastroenterology,MGH for Children, 3Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Summary

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Neutrophil trans-epithelial migration in response to mucosal bacterial infection contributes to epithelial injury and clinical disease. An in vitro model has been developed that combines pathogen, human neutrophils, and polarized human epithelial cell layers grown on transwell filters to facilitate investigations towards unraveling the molecular mechanisms orchestrating this phenomenon.

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Kusek, M. E., Pazos, M. A., Pirzai, W., Hurley, B. P. In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration. J. Vis. Exp. (83), e50823, doi:10.3791/50823 (2014).

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