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Floresan Aktive Edilmiş Çekirdekler Ayıklama Kullanılarak Yetişkin İnsan Astrosit Popülasyonlarının Taze Dondurulmuş Korteksten İzolasyonu
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
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Floresan Aktive Edilmiş Çekirdekler Ayıklama Kullanılarak Yetişkin İnsan Astrosit Popülasyonlarının Taze Dondurulmuş Korteksten İzolasyonu

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3,130 Views

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08:18 min

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April 16, 2021

DOI:

10.3791/62405-v

DOI:
10.3791/62405-v

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08:18 min
April 16, 2021

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, , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science,Fudan University, 4Department of Psychiatry,Icahn School of Medicine at Mount Sinai

Summary

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İnsan astrosit popülasyonlarını aşağı akış moleküler analizlerinde kullanılmak üzere taze dondurulmuş dokudan zenginleştiren ve izole eden bir yöntem geliştirdik.

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Mussa, Z., Tome-Garcia, J., Jiang, Y., Akbarian, S., Tsankova, N. M. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (170), e62405, doi:10.3791/62405 (2021).

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