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Isolamento di popolazioni di astrociti umani adulti dalla corteccia appena congelata mediante selezione dei nuclei attivati dalla fluorescenza
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
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Isolamento di popolazioni di astrociti umani adulti dalla corteccia appena congelata mediante selezione dei nuclei attivati dalla fluorescenza

italiano

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3,120 Views

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08:18 min

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April 16, 2021

DOI:

10.3791/62405-v

DOI:
10.3791/62405-v

•

08:18 min
April 16, 2021

•

7 Views
, , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science,Fudan University, 4Department of Psychiatry,Icahn School of Medicine at Mount Sinai

Summary

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Abbiamo sviluppato un metodo che arricchisce e isola le popolazioni di astrociti umani da tessuti freschi congelati per l'uso in analisi molecolari a valle.

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Mussa, Z., Tome-Garcia, J., Jiang, Y., Akbarian, S., Tsankova, N. M. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (170), e62405, doi:10.3791/62405 (2021).

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