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蛍光活性化核選別を用いた新鮮凍結皮質からの成人ヒトアストロサイト集団の単離
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
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蛍光活性化核選別を用いた新鮮凍結皮質からの成人ヒトアストロサイト集団の単離

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3,130 Views

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08:18 min

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April 16, 2021

DOI:

10.3791/62405-v

DOI:
10.3791/62405-v

•

08:18 min
April 16, 2021

•

7 Views
, , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science,Fudan University, 4Department of Psychiatry,Icahn School of Medicine at Mount Sinai

Summary

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我々は、下流の分子分析に使用するために、ヒトアストロサイト集団を新鮮凍結組織から濃縮し、単離する方法を開発しました。

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Cite this Article

Mussa, Z., Tome-Garcia, J., Jiang, Y., Akbarian, S., Tsankova, N. M. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (170), e62405, doi:10.3791/62405 (2021).

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