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Isolamento de populações de astúcitos humanos adultos do córtex congelado fresco usando a classificação de núcleos ativados por fluorescência
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Isolamento de populações de astúcitos humanos adultos do córtex congelado fresco usando a classificação de núcleos ativados por fluorescência

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3,130 Views

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08:18 min

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April 16, 2021

DOI:

10.3791/62405-v

DOI:
10.3791/62405-v

•

08:18 min
April 16, 2021

•

7 Views
, , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science,Fudan University, 4Department of Psychiatry,Icahn School of Medicine at Mount Sinai

Summary

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Desenvolvemos um método que enriquece e isola populações de astrócitos humanos de tecido fresco congelado para uso em análises moleculares a jusante.

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Mussa, Z., Tome-Garcia, J., Jiang, Y., Akbarian, S., Tsankova, N. M. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (170), e62405, doi:10.3791/62405 (2021).

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