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Isolement de populations d’astrocytes humains adultes à partir de cortex fraîchement congelés à l’aide du tri des noyaux activés par fluorescence
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
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Isolement de populations d’astrocytes humains adultes à partir de cortex fraîchement congelés à l’aide du tri des noyaux activés par fluorescence

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3,120 Views

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08:18 min

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April 16, 2021

DOI:

10.3791/62405-v

DOI:
10.3791/62405-v

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08:18 min
April 16, 2021

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7 Views
, , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science,Fudan University, 4Department of Psychiatry,Icahn School of Medicine at Mount Sinai

Summary

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Nous avons développé une méthode qui enrichit et isole les populations d’astrocytes humains à partir de tissus fraîchement congelés pour une utilisation dans les analyses moléculaires en aval.

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Cite this Article

Mussa, Z., Tome-Garcia, J., Jiang, Y., Akbarian, S., Tsankova, N. M. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (170), e62405, doi:10.3791/62405 (2021).

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