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Microscopia Multifotol Intravital em Tempo Real para visualizar tratamentos focados de ultrassom e microbolhas para aumentar a permeabilidade da barreira hematoencefálica
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February 05, 2022
DOI:
1Physical Sciences Platform,Sunnybrook Research Institute, 2Institute of Biomedical Engineering,University of Toronto, 3Department of Physics,Norwegian University of Science and Technology, 4Department of Clinical Medicine,University of Bergen, 5Exact Therapeutics AS, 6Department of Medical Biophysics,University of Toronto
Summary
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Este protocolo descreve os procedimentos cirúrgicos e técnicos que permitem imagens de fluorescência multifotona em tempo real do cérebro de roedores durante tratamentos de ultrassom e microbolhas focados para aumentar a permeabilidade da barreira hematoencefálica.
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Poon, C., Mühlenpfordt, M., Olsman, M., Kotopoulis, S., de Lange Davies, C., Hynynen, K. Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability. J. Vis. Exp. (180), e62235, doi:10.3791/62235 (2022).
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