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Microscopia multifotonica intravitale in tempo reale per visualizzare trattamenti a ultrasuoni e microbolle focalizzati per aumentare la permeabilità della barriera emato-encefalica
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February 05, 2022
DOI:
1Physical Sciences Platform,Sunnybrook Research Institute, 2Institute of Biomedical Engineering,University of Toronto, 3Department of Physics,Norwegian University of Science and Technology, 4Department of Clinical Medicine,University of Bergen, 5Exact Therapeutics AS, 6Department of Medical Biophysics,University of Toronto
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Questo protocollo descrive le procedure chirurgiche e tecniche che consentono l'imaging a fluorescenza multifotonica in vivo in tempo reale del cervello dei roditori durante i trattamenti a ultrasuoni e microbolle focalizzati per aumentare la permeabilità della barriera emato-encefalica.
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Poon, C., Mühlenpfordt, M., Olsman, M., Kotopoulis, S., de Lange Davies, C., Hynynen, K. Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability. J. Vis. Exp. (180), e62235, doi:10.3791/62235 (2022).
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