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Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability
JoVE 杂志
生物工程
Author Produced
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JoVE 杂志 生物工程
Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

DOI:
10.3791/62235-v

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06:29 min

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February 05, 2022

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, , , , ,
1Physical Sciences Platform,Sunnybrook Research Institute, 2Institute of Biomedical Engineering,University of Toronto, 3Department of Physics,Norwegian University of Science and Technology, 4Department of Clinical Medicine,University of Bergen, 5Exact Therapeutics AS, 6Department of Medical Biophysics,University of Toronto

Chapters

  • 00:07Introduction
  • 01:18Material Preparation
  • 01:46Animal Preparation
  • 03:26Placement of the Ring Transducer
  • 04:05Multiphoton Microscopy Imaging
  • 04:30Ultrasound Exposure
  • 04:56Representative Results
  • 05:52Conclusion

Summary

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This protocol describes the surgical and technical procedures that enable real-time in vivo multiphoton fluorescence imaging of the rodent brain during focused ultrasound and microbubble treatments to increase blood-brain barrier permeability.

Tags

Artificial IntelligenceBlood-brain BarrierMicrobubblesMultiphoton MicroscopyUltrasound

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