Dergi
/
Biyomühendislik
/
Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability
JoVE Journal
Biyomühendislik
Author Produced
This content is Free Access.
JoVE Journal Biyomühendislik
Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

DOI:
10.3791/62235-v

•

06:29 min

•

February 05, 2022

•

, , , , ,
1Physical Sciences Platform,Sunnybrook Research Institute, 2Institute of Biomedical Engineering,University of Toronto, 3Fizik Bölümü,Norwegian University of Science and Technology, 4Department of Clinical Medicine,University of Bergen, 5Exact Therapeutics AS, 6Department of Medical Biophysics,University of Toronto

Bölümler

  • 00:07Introduction
  • 01:18Material Preparation
  • 01:46Animal Preparation
  • 03:26Placement of the Ring Transducer
  • 04:05Multiphoton Microscopy Imaging
  • 04:30Ultrasound Exposure
  • 04:56Representative Results
  • 05:52Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes the surgical and technical procedures that enable real-time in vivo multiphoton fluorescence imaging of the rodent brain during focused ultrasound and microbubble treatments to increase blood-brain barrier permeability.

Etiketler

Artificial IntelligenceBlood-brain BarrierMicrobubblesMultiphoton MicroscopyUltrasound

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

Real-time Monitoring of High Intensity Focused Ultrasound (HIFU) Ablation of In Vitro Canine Livers Using Harmonic Motion Imaging for Focused Ultrasound (HMIFU)

In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Lucifer Yellow – A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How “Molecular Surgery” of the Backbone Affects Folding and Stability

Read Article