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Developmental Biology
/
建立高通量表皮球体培养系统,以模拟角膜细胞干细胞可塑性
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
Please note that all translations are automatically generated.
Click here for the English version.
建立高通量表皮球体培养系统,以模拟角膜细胞干细胞可塑性
DOI:
10.3791/62182-v
•
10:03 min
•
January 30, 2021
•
Yvon Woappi
2
,
Geraldine Ezeka
,
Justin Vercellino
,
Sean M. Bloos
,
Kim E. Creek
,
Lucia Pirisi
1
Department of Pathology, Microbiology and Immunology
,
University of South Carolina School of Medicine
,
2
Department of Dermatology
,
Brigham and Women’s Hospital, Harvard Medical School
,
3
Department of Biochemistry and Molecular Biology
,
University of Maryland School of Medicine Baltimore
,
4
Department of Pathology
,
Albert Einstein College of Medicine
,
5
Department of Pediatrics
,
Vanderbilt University Medical Center
,
6
Department of Drug Discovery and Biomedical Sciences, College of Pharmacy
,
University of South Carolina
Chapters
00:04
Introduction
00:27
Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
02:37
Generating Skin Epidermosphere Cultures in Vitro
05:02
Epidermal Spheroid Re-Plating Assay
05:42
Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
08:06
Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
09:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了在3D悬架培养中系统地培养表皮球体的协议。本协议具有广泛的应用,用于各种上皮组织类型和几个人类疾病和条件的建模。
Tags
Epidermal Spheroid
Keratinocyte Stem Cell
High Throughput Culture
Neonatal Foreskin
Dispase
Trypsin-EDTA
KSFM-SCM Medium
Agarose
96-well Plate
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