Here we describe a protocol for the systematic cultivation of epidermal spheroids in 3D suspension culture. This protocol has wide-ranging applications for use in a variety of epithelial tissue types and for the modeling of several human diseases and conditions.
Woappi, Y., Ezeka, G., Vercellino, J., Bloos, S. M., Creek, K. E., Pirisi, L. Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity. J. Vis. Exp. (167), e62182, doi:10.3791/62182 (2021).