/
/
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:00SA-β-Gal Staining of Frozen TA Muscle Section
- 02:17Immunohistochemistry Using Anti-Nanog Antibody
- 04:38Analysis and Quantification
- 07:30Results: Evaluation of In Vivo Reprogramming After Muscle Injury
- 08:16Conclusion
Here we present a detailed protocol to detect both senescent and pluripotent stem cells in the skeletal muscle upon injury while inducing in vivo reprogramming. This method is suitable for evaluating the role of cellular senescence during tissue regeneration and reprogramming in vivo.
