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Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

DOI:
10.3791/53062-v

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15:40 min

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November 11, 2015

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, ,
1Department of Cell and Developmental Biology,University of Colorado Denver

Chapters

  • 00:05Title
  • 00:48Construction of a Gene-trapped Embryonic Stem (ES) Cell Library
  • 06:11Replication of the ES Cell Library
  • 09:49Stress Selection and Isolation of Resistant Clones
  • 13:14Results: Forward Genetic Screen for Stress Resistance Mutations
  • 14:47Conclusion

Summary

Automatic Translation

Automatic Translation

Stress resistance is one of the hallmarks for longevity and is known to be genetically governed. Here, we developed an unbiased high-throughput method to screen for mutations that confer stress resistance in ES cells with which to develop mouse models for longevity studies.

Tags

Genetic ScreenForward GeneticsEmbryonic Stem CellsHigh-throughputStress ResistanceParaquatOxidative StressMouse ModelsGene Function

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