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Journal
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Biochemistry
/
体外荧光显微镜基于动感检测的肌素特异性适应
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
Please note that all translations are automatically generated.
Click here for the English version.
体外荧光显微镜基于动感检测的肌素特异性适应
DOI:
10.3791/62180-v
•
08:57 min
•
February 04, 2021
•
Ananya Tripathi
,
Charles Bond
2
,
James R. Sellers
,
Neil Billington
,
Yasuharu Takagi
1
Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute
,
National Institutes of Health
,
2
Department of Physiology, Perelman School of Medicine
,
University of Pennsylvania
Chapters
00:05
Introduction
01:04
Gliding Actin Filament Assay
05:02
Performing the Myosin 5a TIRF Microscopy Assay
06:40
Results: Gliding Activity Assay of the NM2b and M5a-HMM Filaments
08:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一个过程来表达和净化肌素5a,然后讨论其特征,使用合奏和单分子体外荧光显微镜为基础的测定,以及如何修改这些方法来描述非肌肉肌素2b。
Tags
Myosin
In Vitro
Fluorescence Microscopy
Motility Assays
Non-muscle Myosin
Protein Dynamics
Single Molecule
Ensemble
Cytoskeletal Systems
Kinesins
Dyneins
Microtubules
Nitrocellulose
Flow Chamber
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