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Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

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08:57 min

February 04, 2021

DOI:

08:57 min
February 04, 2021

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Summary

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Presented here is a procedure to express and purify myosin 5a followed by a discussion of its characterization, using both ensemble and single molecule in vitro fluorescence microscopy-based assays, and how these methods can be modified for the characterization of nonmuscle myosin 2b.

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